Solubility: Soluble in 50% acetic acid in water (5 mg/ml), DMF, DMSO, methanol, and slightly soluble water (0.4 mg/ml).
Ac-DEVD-AFC is a fluorogenic substrate for caspase-3. Caspase-3 is a protease that is rapidly activated during apoptosis and that cleaves several substrates, including poly(ADP-ribose) polymerase (PARP). Cleavage of Ac-DEVDAFC is monitored by measuring an increase in fluorescence signal due to AFC cleavage. The excitation and emission maxima for AFC are 400 nm and 505 nm, respectively.
Each peptide comes complete with the following information:
a QC summary sheet
two reverse phase HPLC profiles to verify purity
mass spectrometry to verify molecular weight
amino acid analysis to verify composition
We use outside laboratories to provide the amino acid analysis and mass spectrometry. This third-party verification allows us to remain objective and has helped us achieve an excellent record of delivering top quality peptides since 1993.